Journal: Journal of Cancer

Article Title: Curcumin Enhances the Anticancer Effect Of 5-fluorouracil against Gastric Cancer through Down-Regulation of COX-2 and NF- κB Signaling Pathways

doi: 10.7150/jca.20196

Figure Lengend Snippet: Inhibitory effects of 5-FU (A), and curcumin (B) alone or in combination (C) on MKN45, AGS and GES-1 cells for 48 h or 72 h incubation. The results are representative of at least three independent experiments run in triplicate.

Article Snippet: Human gastric cancer cells MKN45 and AGS and normal gastric mucosal cells GES-1 were purchased from China Center for Type Culture Collection (CCTCC, Wuhan, Hubei, China).

Techniques: Incubation

Journal: Journal of Cancer

Article Title: Curcumin Enhances the Anticancer Effect Of 5-fluorouracil against Gastric Cancer through Down-Regulation of COX-2 and NF- κB Signaling Pathways

doi: 10.7150/jca.20196

Figure Lengend Snippet: Exponential effect curves of the combination of 5-FU and curcumin in MKN45, AGS and GES-1 cells for 48 h or 72 h treatment. CI: combination index; Fa was the combination effect of two drugs. When CI > 1, antagonist effect; CI = 1, additive effect; and CI < 1, synergistic effect. Each point is corresponding to a CI value of different drug combination effects. The results are representative of at least three independent experiments run in triplicate.

Article Snippet: Human gastric cancer cells MKN45 and AGS and normal gastric mucosal cells GES-1 were purchased from China Center for Type Culture Collection (CCTCC, Wuhan, Hubei, China).

Techniques:

Journal: Journal of Cancer

Article Title: Curcumin Enhances the Anticancer Effect Of 5-fluorouracil against Gastric Cancer through Down-Regulation of COX-2 and NF- κB Signaling Pathways

doi: 10.7150/jca.20196

Figure Lengend Snippet: Effects of 5-FU and curcumin alone or in combination on the protein expressions of COX-2 and NF-κB in MKN45 cells by Western blotting analysis. MKN45 cells were treated with 5-FU and curcumin alone or in combination for 48 h. A: Band 1 was COX-2 and NF-κB proteins and β-actin (control); Band 2 was 25 μmol/l of curcumin; Band 3 was 50 μmol/l of 5-FU; Band 4 was 25 μmol/l of 5-FU + 12.5 μmol/l of curcumin; and band 5 was 50 μmol/l of 5-FU + 25 μmol/l of curcumin. B: The ratio of protein expressions of COX-2 and NF-κB. The results are representative of at least three independent experiments run in triplicate and expressed as the mean ± SD. * P < 0.05 vs control; ** P < 0.05 vs 5-FU and curcumin alone.

Article Snippet: Human gastric cancer cells MKN45 and AGS and normal gastric mucosal cells GES-1 were purchased from China Center for Type Culture Collection (CCTCC, Wuhan, Hubei, China).

Techniques: Western Blot, Control

Journal: Journal of Cancer

Article Title: Curcumin Enhances the Anticancer Effect Of 5-fluorouracil against Gastric Cancer through Down-Regulation of COX-2 and NF- κB Signaling Pathways

doi: 10.7150/jca.20196

Figure Lengend Snippet: Antitumor efficacy and toxicity of 5-FU and curcumin alone or in combination in nude mice bearing advanced human gastric cancer MKN45 tumor xenografts in vivo . A: Kinetics of tumor volume (mm 3 ) in nude mice treated with 5-FU and curcumin alone or in combination; B: Kinetics of body weight change in nude mice treated with 5-FU and curcumin alone or in combination; C: Tumor weight (g) of MKN45 xenografts bearing in nude mice treated with 5-FU and curcumin alone or in combination on day 18 when the mice were humanely sacrificed; D: Tumor inhibitory rates (%) of MKN45 xenografts bearing in nude mice treated with 5-FU and curcumin alone or in combination on day 18, the data were calculated from actually tumor weight. Treatments were initiated 10 days after cultured MKN45 cells were inoculated subcutaneously (s.c.) in nude mice when the tumors reached to ~100 mm 3 (mg) by i.p. injection with 0.2 ml of normal saline (as control), 5-FU (52 mg/kg), curcumin (74 mg/kg), or 5-FU (52 mg/kg) + curcumin (74 mg/kg), respectively. The mice were treated every three days (day 0, 3, 6, 9, 12, and 15) for total of 6 times and were humanely sacrificed on day 18 when the tumors in the control mice reached to near 2000 mm 3 (mg). Eight mice were used for each group and the data were presented as mean ± SD.

Article Snippet: Human gastric cancer cells MKN45 and AGS and normal gastric mucosal cells GES-1 were purchased from China Center for Type Culture Collection (CCTCC, Wuhan, Hubei, China).

Techniques: In Vivo, Cell Culture, Injection, Saline, Control